(2 references)-
- 抗原 See all Myoglobin (MB) ELISA试剂盒
- Myoglobin (MB)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- The Myoglobin Blood Test ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The myoglobin assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the myoglobin molecule. Mouse monoclonal anti-myoglobin antibody is used for solid phase immobilization (on the microtiter wells). A goat anti-myoglobin antibody is in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the two antibodies, resulting in the myoglobin molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 45 minute incubation at room temperature, the wells are washed with water to remove unbound labeled antibodies. A TMB (Tetramethyl-benzidine) Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of myoglobin is directly proportional to the color intensity of the myoglobin blood test sample. Absorbance is measured spectrophotometrically at 450 nm.
- 样品类型
- Blood
- Analytical Method
- Quantitative
- Featured
- Discover our best selling MB ELISA Kit
- Top Product
- Discover our top product MB ELISA Kit
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Myoglobin (MB) ELISA Kit
MB 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Myoglobin (MB) ELISA KitMB 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Myoglobin (MB) ELISA KitMB 适用: 小鼠 Colorimetric Sandwich ELISA 9.375 ng/mL - 300 ng/mL Plasma, Serum, Urine
Myoglobin (MB) ELISA KitMB 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 200 ng/mL Plasma, Serum, Urine
Myoglobin (MB) ELISA KitMB 适用: 兔 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Myoglobin (MB) ELISA KitMB 适用: 猴 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Myoglobin (MB) ELISA KitMB 适用: 豚鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Myoglobin (MB) ELISA KitMB 适用: Cow Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
Myoglobin (MB) ELISA KitMB 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Myoglobin (MB) ELISA KitMB 适用: Pig Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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: "TNF-? and TNFR1 responses to recovery therapies following acute resistance exercise." in: Frontiers in physiology, Vol. 6, pp. 48, (2015) (PubMed).
: "The impact of an ultramarathon on hormonal and biochemical parameters in men." in: Wilderness & environmental medicine, Vol. 25, Issue 3, pp. 278-88, (2014) (PubMed).
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: "TNF-? and TNFR1 responses to recovery therapies following acute resistance exercise." in: Frontiers in physiology, Vol. 6, pp. 48, (2015) (PubMed).
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- 抗原 See all Myoglobin (MB) ELISA试剂盒
- Myoglobin (MB)
- 别名
- Myoglobin (MB 产品)
- 别名
- PVALB ELISA Kit, AI325109 ELISA Kit, zgc:65819 ELISA Kit, zgc:77764 ELISA Kit, MB ELISA Kit, DKFZp468H096 ELISA Kit, myg ELISA Kit, mb ELISA Kit, MYF4 ELISA Kit, bHLHc3 ELISA Kit, myo ELISA Kit, Myoglobin ELISA Kit, myoglobin ELISA Kit, myogenin ELISA Kit, MB ELISA Kit, Mb ELISA Kit, mb ELISA Kit, myg ELISA Kit, Myog ELISA Kit
- 背景
- Myoglobin, a heme protein with a molecular weight of approximately 17,500 Daltons is found in both cardiac and skeletal muscle. Damage to either type of muscle following conditions such as trauma, ischemia, and diseases that cause myopathy, is associated with the release of myoglobin into serum. Specifically, following cardiac necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Myoglobin levels increase measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours. In the absence of skeletal muscle trauma or other factors associated with a non-cardiac related increase in circulating myoglobin, its levels have been used as an early marker for myocardial infarct. A number of reports suggest using the measurement of myoglobin as a diagnostic aid in ruling out myocardial infarction with negative predictive values of up to 100% reported at certain time periods after the onset of symptoms.9-15 Unlike the other cardiac enzymes such as creeatine kinase and the MB isoform (i.e., CK and CKMB) which do not reach serum levels until several hours post-infarction (approx. 19 hours), myoglobin levels can be expected to peak within 6 to 9 hours. The Myoglobin Enzyme Immunoassay provides a rapid, sensitive, and reliable myoglobin assay for the quantitative measurement of myoglobin in serum. The antibodies developed for the test will determine a minimal concentration of 5.0 ngml, and there is no cross-reactivity with related cardiac or skeletal enzymes.
- 途径
- Brown Fat Cell Differentiation
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