Anti-Brucella IgG Antibody ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 Anti-Brucella IgG Antibody.
产品编号 ABIN1326797
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Room 801-803
Quick Overview for Anti-Brucella IgG Antibody ELISA 试剂盒 (ABIN1326797)
抗原
Anti-Brucella IgG Antibody
适用
人
检测方法
Colorimetric
实验类型
Competition ELISA
应用范围
ELISA
样品类型
Serum
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原理
- Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.
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Analytical Method
- Qualitative
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Anti-Brucella IgG Antibody
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别名
- Brucella IgG
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物质类
- Antibody, Antibody
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背景
- Brucella is a gram negative coccobacilli capable of infecting a wide range of animal and man. Of the three species causing human infection, iB. melitensisi is the most patogenic followed by iB. suisi and iB. abortusi . Brucellosis is transmitted through contaminated and untreated milk and milk products and by direct contact with infected animals (cattle, sheep, goats, pigs, camels, buffaloes, and, very recently, seals), animal carcasses, and abortion materials. Worldwide, millions of inidual are at risk, especially in developing countries where the infection in animals has not been brought under control, heat treatment procedures of milk (e.g. pasteurization) are not routinely applied, and food habits such as consumption of raw milk.The incubation period of brucellosis is usually one to three weeks, but sometimes may be several months. The illness may be mild and self-limiting or severe. The disease is accompanied by continued, intermittent, or irregular fever, headache, weight loss and generalized aching and fatigue. Urogenital symptoms may dominate the clinical presentation in some patients. This method uses iB. abortus iouter membrane, which is shared by the other species. Brucella IgG and IgA antibodies persist for many years after infection. A significant increase in Brucella IgG level is in patients with symptoms of brucellosis is indicative of recent exposure. IgM antibodies are present in acute brucellosis and also found in about 33% of patients with chronic brucellosis.
抗原
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