TOLLIP ELISA 试剂盒
The 人 TOLLIP ELISA Kit (ABIN1161909) is a Colorimetric ELISA Kit designed to quantify 人 TOLLIP.
产品编号 ABIN1161909
发货至:
中国
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中国
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Quick Overview for TOLLIP ELISA 试剂盒 (ABIN1161909)
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TOLLIP
(Toll Interacting Protein (TOLLIP))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
31.2-2000 pg/mL
应用范围
ELISA
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最低检测浓度
- 31.2 pg/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of human Toll-interacting protein, concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural human. No significant cross-reactivity or interference was observed.
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组件
- Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)
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Toll Interacting Protein (TOLLIP) ELISA Kit
TOLLIP 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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说明
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Gene Name: TOLLIP
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- TOLLIP (Toll Interacting Protein (TOLLIP))
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别名
- Toll-interacting protein
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背景
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Synonyms: Homo sapiens,Human,Toll-interacting protein,TOLLIP
Subunit: Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2.
Function: Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. -
UniProt
- Q9H0E2
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