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PPT1 ELISA 试剂盒

The 人 PPT1 ELISA Kit (ABIN1146897) is a Colorimetric ELISA Kit designed to quantify 人 PPT1.
产品编号 ABIN1146897
发货至: 中国
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Quick Overview for PPT1 ELISA 试剂盒 (ABIN1146897)

抗原

See all PPT1 ELISA试剂盒
PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))

适用

  • 3
  • 1
  • 1
  • 1

检测方法

Colorimetric

检测范围

0.62-40.0 ng/mL

应用范围

ELISA
  • 最低检测浓度

    0.62 ng/mL

    产品特性

    The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPT-1. During the reaction, PPT-1 in the sample or standard competes with a fixed amount of biotin-labeled PPT-1 for sites on a pre-coated Monoclonal antibody specific to PPT-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PPT-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    组件

    Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)
  • 说明

    Gene Name: PPT1

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C

    储存方法

    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • 抗原 See all PPT1 ELISA试剂盒

    PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))

    别名

    Palmitoyl-protein thioesterase 1

    背景

    Synonyms: Homo sapiens,Human,Palmitoyl-protein hydrolase 1,Palmitoyl-protein thioesterase 1,PPT,PPT1,PPT-1

    途径

    SARS-CoV-2 Protein Interactome
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