ADNP ELISA 试剂盒
Quick Overview for ADNP ELISA 试剂盒 (ABIN1120362)
抗原
See all ADNP ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of mouseActivity-dependent neuroprotector homeobox proteinconcentrationsin serum, plasma, tissue homogenates,cell culture supernates, and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural MouseActivity-dependent neuroprotector homeobox protein. No significant cross-reactivity or interference was observed.
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组件
- Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x60µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)
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Activity-Dependent Neuroprotector Homeobox (ADNP) ELISA Kit
ADNP 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Activity-Dependent Neuroprotector Homeobox (ADNP) ELISA KitADNP 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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说明
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Gene Name: Adnp
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- he ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activity-dependent neuroprotector homeobox protein, during the reaction,Activity-dependent neuroprotector homeobox proteinin the sample or standard competes with a fixed amount of biotin-labeled Activity-dependent neuroprotector homeobox proteinfor sites on a pre-coated Monoclonal antibody specific to Activity-dependent neuroprotector homeobox protein. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentrationof Activity-dependent neuroprotector homeobox proteinin the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- ADNP (Activity-Dependent Neuroprotector Homeobox (ADNP))
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别名
- Activity-dependent neuroprotector homeobox protein
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背景
- Synonyms: Activity-dependent neuroprotective protein,Activity-dependent neuroprotector homeobox protein,Adnp,Mouse,Mus musculus
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途径
- Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process
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