IgG ELISA 试剂盒
Quick Overview for IgG ELISA 试剂盒 (ABIN1113184)
抗原
See all IgG ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 16 ng/mL
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原理
- Rapid quantification of IgG in cell culture supernatant. Screening of higher producing clones, follow-up of production process, Pharmacokinetic studies
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品牌
- FastELISA®
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Analytical Method
- Quantitative
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特异性
- The method enables the detection of natural human IgG as well as recombinant humanized or fully human monoclonal antibodies. Chimeric (human-mouse) recombinant antibodies are detected with the method
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交叉反应 （详细）
- < 1% for the following IgG: Mouse, Cow, Horse, Sheep and Swine and are < 2 % for Rabbit IgG.
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灵敏度
- 4 ng/mL
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产品特性
- The human immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of human IgG in cell culture supernatant and serum.
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IgG ELISA Kit
适用: 兔 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Rabbit TrueBlot® Set (with IP beads)WB, IP 适用: 兔
Mouse TrueBlot® Set (with IP beads)WB, IP 适用: 小鼠
IgG ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: 人 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鸡 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: Pig Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
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说明
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One Step ELISA 30 min total time
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实验时间
- < 1 h
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板类型
- Pre-coated
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实验流程
- A polyclonal antibody specific to the gamma heavy chain of human IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and human IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-human IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of human IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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试剂准备
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dilution
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限制
- 仅限研究用
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储存条件
- 4 °C
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有效期
- 12 months
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- IgG
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别名
- Immunoglobulin G
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物质类
- Antibody
抗原 See all IgG ELISA试剂盒
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