CCL5 ELISA 试剂盒
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北京 101111
Quick Overview for CCL5 ELISA 试剂盒 (ABIN1112783)
抗原
See all CCL5 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- For quantitative detection of Rantes in Human serum, plasma, tissue lysatesor cell culture supernatants.
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Analytical Method
- Quantitative
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灵敏度
- < 3 pg/mL
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组件
- 1. One 96-well plate pre-coated with anti-Human Rantes antibody 2. Lyophilized Human Rantes standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human Rantes antibody (Concentrated): 130 µl.
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试剂未包括
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- 37 °C incubator
- Microplate reader (wavelength: 450 nm)
- Precise pipette and disposable pipette tips
- Automated plate washer
- ELISA shaker
- 1.5 mL of Eppendorf tubes
- Plate cover
- Absorbent filter papers
- Plastic or glass container with volume of above 1 L
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Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA Kit
CCL5 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: Pig Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 豚鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 小鸡 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 豚鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rantes polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rantes polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the Rantes amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of Rantes can be calculated.
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板类型
- Pre-coated
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试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37 °C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
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样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysates and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin, EDTA or citrate as the anticoagulant. Centrifuge for 15 min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. -
限制
- 仅限研究用
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储存液
- Sodium azide, Thimerosal (Merthiolate)
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 2-8°C for 6 months, or at -20°C for 12 months.
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有效期
- 12 months
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- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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别名
- RANTES
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背景
- Rantes, also known as Chemokine (C-C motif) ligand 5 (also CCL5), is a protein which in humans is encoded by the CCL5 gene, this chemokine has been localized to chromosome 17 in humans. Rantes is an 8kDa protein classified as a chemotactic cytokine or chemokine. It is chemotactic for T cells, eosinophils, and basophils. The Rantes plays an active role in recruiting leukocytes into inflammatory sites. With the help of particular cytokines (i.e., IL-2 and IFN-gamma) that are released by T cells, CCL5 also induces the proliferation and activation of certain natural-killer (NK) cells to form CHAK (CC-Chemokine-activated killer) cells. It is also an HIV-suppressive factor released from CD8+ T cells. Recently, the RANTES protein has been engineered for in vivo production by Lactobacillus bacteria, and this solution is being developed into a possible HIV entry-inhibiting topical microbicide.
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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