IL-5 ELISA 试剂盒
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- 抗原 See all IL-5 (IL5) ELISA试剂盒
- IL-5 (IL5) (Interleukin 5 (IL5))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- For quantitative detection of IL-5 in human serum, body fluids, tissue lysate or cell culture supernate.
- 样品类型
- Cell Culture Supernatant, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 灵敏度
- < 2 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-Human IL-5 antibody 2. Lyophilized Human IL-5 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human IL-5 antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
- Featured
- Discover our best selling IL5 ELISA Kit
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- Discover our top product IL5 ELISA Kit
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Interleukin 5 (IL5) ELISA Kit
IL5 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 豚鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 山羊 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 绵羊 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 马 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 小鸡 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-5 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-5 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the IL-5 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of IL-5 can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysate or cell culture supernate: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C.
Serum: Coagulate the serum at room temperature (about 4 hours) or coat at 4°C overnight. Centrifuge at approximately 2000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20 °C . Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. 3. The sample with hyperlipoidemia and haemolyticus is not suitable for this kit. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all IL-5 (IL5) ELISA试剂盒
- IL-5 (IL5) (Interleukin 5 (IL5))
- 别名
- IL-5 (IL5 产品)
- 别名
- EDF ELISA Kit, IL-5 ELISA Kit, TRF ELISA Kit, Il-5 ELISA Kit, IL5 ELISA Kit, interleukin 5 ELISA Kit, IL5 ELISA Kit, Il5 ELISA Kit
- 背景
- Interleukin 5 (IL-5) is an interleukin produced by T helper-2 cells and mast cells. It is a 115-amino acid-long TH2 cytokine that is part of the hematopoietic family. It is expressed by eosinophils and has been observed in the mast cells of asthmatic airways by immunohistochemistry. IL-5 expression is regulated by several transcription factors including GATA3. IL-5 is a key mediator in eosinophil activation. It has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma, where in a large increase in the number of circulating, airway tissue, and induced sputum eosinophils have been observed. Given the high concordance of eosinophils and, in particular, allergic asthma pathology, it has been widely speculated that eosinophils have an important role in the pathology of this disease.
- 途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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