IGFBPI ELISA 试剂盒
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- 抗原 See all IGFBPI ELISA试剂盒
- IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- For quantitative detection of IGFBP-1 in mouse serum, body fluids, tissue lysates or cell culture supernatants.
- 样品类型
- Cell Culture Supernatant, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 灵敏度
- < 3 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-mouse IGFBP-1 antibody 2. Lyophilized Mouse IGFBP-1 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse IGFBP-1 antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
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- Discover our top product IGFBPI ELISA Kit
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Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) ELISA Kit
IGFBPI 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) ELISA KitIGFBPI 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) ELISA KitIGFBPI 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) ELISA KitIGFBPI 适用: Pig Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) ELISA KitIGFBPI 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) ELISA KitIGFBPI 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) ELISA KitIGFBPI 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) ELISA KitIGFBPI 适用: Cow Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) ELISA KitIGFBPI 适用: 人 AA 26-259 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Insulin-Like Growth Factor Binding Protein 1 (IGFBPI) ELISA KitIGFBPI 适用: 小鼠 AA 26-272 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IGFBP-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IGFBP-1 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the IGFBP-1 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of IGFBP-1 can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysates and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 2000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C . Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all IGFBPI ELISA试剂盒
- IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))
- 别名
- IGFBP-1 (IGFBPI 产品)
- 别名
- cb656 ELISA Kit, igfbp1 ELISA Kit, IGFBP-1 ELISA Kit, MGC68538 ELISA Kit, afbp ELISA Kit, ibp1 ELISA Kit, pp12 ELISA Kit, igf-bp25 ELISA Kit, higfbp-1 ELISA Kit, IGFBP1 ELISA Kit, LOC100223208 ELISA Kit, LOC100305121 ELISA Kit, AFBP ELISA Kit, IBP1 ELISA Kit, IGF-BP25 ELISA Kit, PP12 ELISA Kit, hIGFBP-1 ELISA Kit, IGFBA ELISA Kit, insulin like growth factor binding protein 1 ELISA Kit, insulin-like growth factor binding protein 1a ELISA Kit, insulin like growth factor binding protein 1 S homeolog ELISA Kit, insulin like growth factor binding protein 1 L homeolog ELISA Kit, insulin-like growth factor binding protein 1 ELISA Kit, IGFBP1 ELISA Kit, igfbp1a ELISA Kit, igfbp1.S ELISA Kit, igfbp1.L ELISA Kit, igfbp1 ELISA Kit, LOC100305121 ELISA Kit, Igfbp1 ELISA Kit
- 背景
- Insulin-like growth factor-binding protein 1 (IGFBP-1) also known as placental protein 12 (PP12) is a member of the Insulin-like growth factor-binding protein (IGFBP) family. It is synthesized in liver, secretory endometrium, and decidua. The IGFBP1 gene has 4 exons, spans 5.9 kb, and mapped to 7p13-p12. This protein has an IGFBP domain and a type-I thyroglobulin domain. It binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. Leu and George (2007) concluded that IGFBP1 is a negative regulator of the p53/BAK-dependent pathway of apoptosis.
- 途径
- Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding
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