Prokineticin 1 ELISA 试剂盒
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- 抗原 See all Prokineticin 1 (Prok1) ELISA试剂盒
- Prokineticin 1 (Prok1)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- For quantitative detection of EG-VEGF in human serum, plasma, or cell culture supernatants.
- 样品类型
- Serum, Plasma, Cell Culture Supernatant
- Analytical Method
- Quantitative
- 灵敏度
- < 7 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-Human EG-VEGF antibody 2. Lyophilized Human EG-VEGF standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human EG-VEGF antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
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Prokineticin 1 (Prok1) ELISA Kit
Prok1 适用: 小鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prokineticin 1 (Prok1) ELISA KitProk1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prokineticin 1 (Prok1) ELISA KitProk1 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prokineticin 1 (Prok1) ELISA KitProk1 适用: Cow Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Prokineticin 1 (Prok1) ELISA KitProk1 适用: 人 AA 20-105 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Prokineticin 1 (Prok1) ELISA KitProk1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-EG-VEGF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-EG-VEGF polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the EG-VEGF amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of EG-VEGF can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin, EDTA or citrate as the anticoagulant. Centrifuge for 10 min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all Prokineticin 1 (Prok1) ELISA试剂盒
- Prokineticin 1 (Prok1)
- 别名
- EG-VEGF (Prok1 产品)
- 别名
- PK1 ELISA Kit, zgc:171925 ELISA Kit, EGVEGF ELISA Kit, PRK1 ELISA Kit, EG-VEGF ELISA Kit, prokineticin 1 ELISA Kit, PROK1 ELISA Kit, prok1 ELISA Kit, Prok1 ELISA Kit
- 背景
- Endocrine gland-derived vascular endothelial growth factor (EG-VEGF) is a human protein encoded by the PROKR1 gene. It induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. EG-VEGF is mitogenic and chemoattractive and able to induce fenestration. Its expression is induced by hypoxia, and there is an HIF1 binding site present on EG-VEGF. It is also able to induce angiogenesis and ovarian cyst formation.
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