BMP7 ELISA 试剂盒
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- 抗原 See all BMP7 ELISA试剂盒
- BMP7 (Bone Morphogenetic Protein 7 (BMP7))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- For quantitative detection of BMP-7 in human serum, plasma, urine, bone tissue or cell culture supernatants.
- 样品类型
- Serum, Plasma, Urine, Tissue Samples, Cell Culture Supernatant
- Analytical Method
- Quantitative
- 灵敏度
- < 10 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-Human BMP-7 antibody 2. Lyophilized Human BMP-7 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human BMP-7 antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
- Featured
- Discover our best selling BMP7 ELISA Kit
- Top Product
- Discover our top product BMP7 ELISA Kit
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Bone Morphogenetic Protein 7 (BMP7) ELISA Kit
BMP7 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 绵羊 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 人 AA 293-431 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Osseous Tissue, Plasma (EDTA), Plasma (heparin), Serum, Urine
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: Hamster AA 292-430 Colorimetric Sandwich ELISA
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 小鼠 AA 292-430 Colorimetric Sandwich ELISA 31.2-2000 pg/mL
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 大鼠 AA 292-430 Colorimetric Sandwich ELISA 31.2-2000 pg/mL
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BMP-7 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BMP-7 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the BMP-7 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of BMP-7 can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Bone Tissue: Extract demineralized bone samples in 4 M Guanidine-HCl and protease inhibitors. Dissolve the final sample in 2 M Guanidine-HCl.
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin or EDTA as the anticoagulant. Centrifuge for 15min at 2-8°C at 1500 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate can not be used as anticoagulant here.
Urine: Aseptically collect the first urine of the day, micturate directly into a sterile container. Remove particular impurities by centrifugation, assay immediately or aliquot and store samples at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all BMP7 ELISA试剂盒
- BMP7 (Bone Morphogenetic Protein 7 (BMP7))
- 别名
- BMP-7 (BMP7 产品)
- 别名
- bmp-7 ELISA Kit, bmp7 ELISA Kit, op-1 ELISA Kit, BMP7 ELISA Kit, zgc:153025 ELISA Kit, OP-1 ELISA Kit, OP1 ELISA Kit, BMP-7 ELISA Kit, fb83f09 ELISA Kit, wu:fb83f09 ELISA Kit, bone morphogenetic protein 7 ELISA Kit, bone morphogenetic protein 7, gene 1 L homeolog ELISA Kit, bone morphogenetic protein 7b ELISA Kit, bone morphogenetic protein 7, gene 2 L homeolog ELISA Kit, bone morphogenetic protein 7a ELISA Kit, BMP7 ELISA Kit, bmp7.1.L ELISA Kit, LOC100136282 ELISA Kit, bmp7b ELISA Kit, Bmp7 ELISA Kit, LOC100008826 ELISA Kit, bmp7.2.L ELISA Kit, bmp7a ELISA Kit
- 背景
- Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a member of the TGF-beta superfamily. It is expressed in the brain, kidneys and bladder. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 induces the phosphorylation of SMAD1 and SMAD5, which in turn induce transcription of numerous osteogenic genes. It has been demonstrated that BMP7 treatment is sufficient to induce all of the genetic markers of osteoblast differentiation in many cell types.
- 途径
- Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance
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