ADIPOQ ELISA 试剂盒
Quick Overview for ADIPOQ ELISA 试剂盒 (ABIN1112554)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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原理
- For quantitative detection of Adiponectin in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
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Analytical Method
- Quantitative
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灵敏度
- < 10 pg/mL
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组件
- 1. One 96-well plate pre-coated with anti-mouse Adiponectin antibody 2. Lyophilized mouse Adiponectin standards: 2 tubes (20ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-mouse Adiponectin antibody (Concentrated): 130 µl.
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试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
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Adiponectin (ADIPOQ) ELISA Kit
ADIPOQ 适用: 人 Colorimetric Sandwich ELISA 1.562-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 绵羊 Colorimetric Competition ELISA 6.25 ng/mL - 100 ng/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 小鸡 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 小鼠 AA 18-247 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate, Urine
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 人 AA 19-244 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate, Urine
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说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Adiponectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Adiponectin polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the Adiponectin amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of Adiponectin can be calculated.
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板类型
- Pre-coated
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试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
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样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysates and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 2000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin, citrate or EDTA as the anticoagulant. Centrifuge for 20 min at 2000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. -
限制
- 仅限研究用
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储存液
- Sodium azide, Thimerosal (Merthiolate)
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- ADIPOQ (Adiponectin (ADIPOQ))
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别名
- Adiponectin / Acrp 30
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背景
- Adiponectin (also referred to as GBP-28, apM1, AdipoQ and Acrp30) is a protein which in humans is encoded by the ADIPOQ gene. This gene spans 16 kb, contains 3 exons, and maps to to chromosome 3q27. Adiponectin is a 244-amino-acid-long polypeptide, and it has four distinct regions. It is a hormone secreted by adipocytes (and also from the placenta in pregnancy) that regulates energy homeostasis and glucose and lipid metabolism. Adipocytes also produce and secrete proteins such as leptin, adipsin, various other complement components and tumor necrosis factor, suggesting a possible link to the immune system.
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