(4 references)-
- 抗原 See all NFkB 试剂盒
- NFkB (Nuclear Factor kappa B (NFkB))
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适用
- 人
- 应用范围
- Activity Assay (AcA), Western Blotting (WB)
- 原理
- Rapid assay to quantitate NF-kappa B activation using cell fractionation into nuclear and cytoplasmic components
- 特异性
- This NF-KB activation assay follows the translocation of the p65 (RelA) subunit of NF-kB into the nucleus, a hallmark of NF-kappa B activation. Cytoplasmic and nuclear fractions are isolated, and Western blots of the fractions are used to quantify the relative p65 concentration. The ratio of nuclear relative to cytoplasmic p65 concentration provides a numerical estimate of NF-kappa B activation.
- 产品特性
- This NF-KB Activation Assay Kit provides all the reagents required to fractionate cells into cytoplasmic and nuclear fractions, as well as a selective and high affinity antibody to detect p65 in Western blots.
- 组件
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- Cytoplasmic Fractionation Reagent (CER-1, 55mL)
- Nuclear Fractionation Reagent (NER-1, 3.5 mL)
- DTT (sufficient to make a 1M solution, volume 64 microliters)
- Rabbit Polyclonal IgG Antibody to p65. (100 µL Use at 1:100 - 1:400 dilution for Western blots)
- Top Product
- Discover our top product NFkB ELISA Kit
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Nuclear Factor kappa B (NFkB) ELISA Kit
NFkB 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitSmall Sample NFkB 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB 适用: 小鸡 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Use at 1:400 dilution for Western blots
- 说明
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Cytoplasmic Fractionation Reagent (55 mL)
Nuclear Fractionation Reagent (3.5 mL)
DTT (sufficient to make a 1 M solution, volume 64 µL) - 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
- 储存方法
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p65Ab: Aliquot, -20°C.
HRPAb: 4°C.
Other components: -20°C
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: "Incorporation of β-sitosterol into the membrane prevents tumor necrosis factor-α-induced nuclear factor-κB activation and gonadotropin-releasing hormone decline." in: Steroids, Vol. 96, pp. 1-6, (2015) (PubMed).
: "Micro-RNA 21 inhibition of SMAD7 enhances fibrogenesis via leptin-mediated NADPH oxidase in experimental and human nonalcoholic steatohepatitis." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 308, Issue 4, pp. G298-312, (2015) (PubMed).
: "Glyoxalase I is differentially expressed in cutaneous neoplasms and contributes to the progression of squamous cell carcinoma." in: The Journal of investigative dermatology, Vol. 135, Issue 2, pp. 589-98, (2015) (PubMed).
: "Combination of ascorbate/epigallocatechin-3-gallate/gemcitabine synergistically induces cell cycle deregulation and apoptosis in mesothelioma cells." in: Toxicology and applied pharmacology, Vol. 274, Issue 1, pp. 35-41, (2013) (PubMed).
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: "Incorporation of β-sitosterol into the membrane prevents tumor necrosis factor-α-induced nuclear factor-κB activation and gonadotropin-releasing hormone decline." in: Steroids, Vol. 96, pp. 1-6, (2015) (PubMed).
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- 抗原
- NFkB (Nuclear Factor kappa B (NFkB))
- 别名
- NF-kappa B (NFkB 产品)
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