(6 references)-
- 适用
- 人
- 检测范围
- 21.9-4378 μmol/L
- 最低检测浓度
- 21.9 μmol/L
- 原理
-
Detection: 480-492 nm, colorimetric assay.
In the PAO assay kit, an easy and convenient method to measure antioxidant capacity is provided. Utilizing the reduction of cupric ion (Cu++ to Cu+), antioxidant capacity of samples can be detected in 5 minutes. Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine) , and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed. PAO can detect not only hydrophilic antioxidants such as Vitamin C, glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. Applicable for assessment of total antioxidants of serum, foods, beverage - 样品类型
- Serum, Food, Beverages
- 组件
-
Standard (Uric acid powder): 1 vial
Sample diluent: 1 bottle
Cu++ solution: 1 bottle
Stop solution: 1 bottle
Micro titer plate: 1 plate
(96 wells) - 试剂未包括
-
A micro plate reader (measuring wavelength 492 nm)
Pipettes and pipette chips
Plastic test tubes
Distilled water
NaOH, HCl solution and pH meter (Not required if standards are prepared with distilled water only).
-
Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Amyloid beta 1-40 (Abeta 1-40) ELISA KitAbeta 1-40 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 适用: 人 Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 小鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 人 AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 人 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
-
- 实验流程
-
1) Prepare 6 levels of standards by diluting 2mM uric acid.
2) Please prepare plastic test tubes for 6 levels of standards and each sample. Pour 390 µL of Sample Diluent, and add 10 µL of standards or diluted samples.
3) Pour 200 µL of mixture to Micro titer plate. Use 200 µL of Sample Diluent for blank well.
4) Read absorbance at 490 nm (as READ1).
5) Add 50 µL of Cu++solution to each well, mix gently, and incubate at room temperature for 3 minutes.
6) Add 50 µL of Stop solution, mix gently, and read absorbance at 490 nm (as READ2).
7) Please draw standard curves by plotting the difference of absorbance readings (READ2 - READ1) as vertical axis, and concentration of uric acid standards (mM) as horizontal axis. Calculate the corresponding uric acid concentration of samples. Multiply corresponding uric acid concentration (mM) of samples by 2189, to estimate antioxidant power (µmol/L). 1mM of uric acid = 2189 µmol/L (copper reducing power) - 限制
- 仅限研究用
-
- 储存条件
- RT
-
-
: "Studies on serum 8-hydroxy guanosine (8-OHdG) as reliable biomarker for psoriasis." in: Journal of the European Academy of Dermatology and Venereology : JEADV, Vol. 27, Issue 5, pp. 655-7, (2013) (PubMed).
: "Evaluation for antioxidant and renoprotective activity of olmesartan using nephrectomy rats." in: Biological & pharmaceutical bulletin, Vol. 32, Issue 12, pp. 2041-5, (2009) (PubMed).
: "Antioxidant effects of flavonoids used as food additives (purple corn color, enzymatically modified isoquercitrin, and isoquercitrin) on liver carcinogenesis in a rat medium-term bioassay." in: Journal of food science, Vol. 73, Issue 7, pp. C561-8, (2008) (PubMed).
: "Oxidative imbalance and cathepsin D changes as peripheral blood biomarkers of Alzheimer disease: a pilot study." in: FEBS letters, Vol. 579, Issue 13, pp. 2759-66, (2005) (PubMed).
: "Antioxidant capacity as a reliable marker of stress in dairy calves transported by road." in: The Veterinary record, Vol. 156, Issue 2, pp. 53-4, (2005) (PubMed).
: "Oxidative stress and its association with coronary artery disease and different atherogenic risk factors." in: Journal of internal medicine, Vol. 256, Issue 4, pp. 308-15, (2004) (PubMed).
-
: "Studies on serum 8-hydroxy guanosine (8-OHdG) as reliable biomarker for psoriasis." in: Journal of the European Academy of Dermatology and Venereology : JEADV, Vol. 27, Issue 5, pp. 655-7, (2013) (PubMed).
-
- 背景
- Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention.
-
You are here:
