Quick Overview for Phospholipase D Assay Kit (ABIN1000328)
抗原
See all Phospholipase D (PLD) 试剂盒
Phospholipase D (PLD)
应用范围
Activity Assay (AcA)
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特异性
- 0.04 U/L
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产品特性
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Sensitive. Use 10 µL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L.
Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening. -
组件
- Assay Buffer: 10 mL. Dye Reagent: 120 µL. Enzyme Mix: 120 µL. Substrate: 1.5 mL. Calibrator: 400 µL.
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试剂未包括
- Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.
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Phospholipase D (PLD) ELISA Kit
PLD 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
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应用备注
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Direct Assays: phospholipase D in biological samples.
Drug Discovery/Pharmacology: effects of drugs on phospholipase D metabolism. -
结果分析
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Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.
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限制
- 仅限研究用
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储存条件
- -20 °C
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- Phospholipase D (PLD)
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别名
- Phospholipase D
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背景
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Quantitative determination of phospholipase D activity by colorimetric (570nm) or fluorimetric (530/590nm) method.
Procedure: 30 min.
Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. This method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.
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