(10 references)
产品编号 ABIN1000327
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for Phospholipd Assay Kit (ABIN1000327)
抗原
Phospholipid
应用范围
Biochemical Assay (BCA)
样品类型
Serum, Plasma (no EDTA)
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产品特性
- Sensitive. Use 20 µL samples. Linear detection range: colorimetric assay 3 - 200 µM, fluorimetric assay 0.6 - 20 µM phospholipid.
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组件
- Assay Buffer: 10 mL. PLD Enzyme: 120 µL. Enzyme Mix: 120 µL. Dye Reagent: 120 µL. Standard: 400 µL 2 mM phosphatidylcholine.
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试剂未包括
- Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.
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应用备注
- Assays: phospholipid in biological samples such as serum and non- EDTA plasma. Drug Discovery/Pharmacology: effects of drugs on choline-containing phospholipid metabolism.
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结果分析
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Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.
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限制
- 仅限研究用
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储存条件
- -20 °C
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: "Drugging a stem cell compartment using Wnt3a protein as a therapeutic." in: PLoS ONE, Vol. 9, Issue 1, pp. e83650, (2014) (PubMed).
: "Liquid crystal droplet-based amplification of microvesicles that are shed by mammalian cells." in: The Analyst, Vol. 139, Issue 10, pp. 2386-96, (2014) (PubMed).
: "Ciprofloxacin encapsulation into giant unilamellar vesicles: membrane binding and release." in: Journal of pharmaceutical sciences, Vol. 102, Issue 2, pp. 694-705, (2013) (PubMed).
: "Decellularization method influences early remodeling of an allogenic tissue scaffold." in: Journal of biomedical materials research. Part A, (2013) (PubMed).
: "Microparticle drug sequestration provides a parallel pathway in the acquisition of cancer drug resistance." in: European journal of pharmacology, Vol. 721, Issue 1-3, pp. 116-25, (2013) (PubMed).
: "Kinetic equivalence of transmembrane pH and electrical potential differences in ATP synthesis." in: The Journal of biological chemistry, Vol. 287, Issue 12, pp. 9633-9, (2012) (PubMed).
: "DNA damage induces down-regulation of UDP-glucose ceramide glucosyltransferase, increases ceramide levels and triggers apoptosis in p53-deficient cancer cells." in: Biochimica et biophysica acta, Vol. 1821, Issue 7, pp. 943-53, (2012) (PubMed).
: "Lysophosphatidic acid activates lipogenic pathways and de novo lipid synthesis in ovarian cancer cells." in: The Journal of biological chemistry, Vol. 287, Issue 30, pp. 24990-5000, (2012) (PubMed).
: "Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury." in: Brain pathology (Zurich, Switzerland), Vol. 22, Issue 1, pp. 41-57, (2011) (PubMed).
: "Liposomes alter thermal phase behavior and composition of red blood cell membranes." in: Biochimica et biophysica acta, Vol. 1808, Issue 1, pp. 474-81, (2010) (PubMed).
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: "Drugging a stem cell compartment using Wnt3a protein as a therapeutic." in: PLoS ONE, Vol. 9, Issue 1, pp. e83650, (2014) (PubMed).
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- Phospholipid
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别名
- Phospholipd
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背景
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Quantitative determination of phospholipid by colorimetric (570nm) or fluorimetric (530/585nm) methods.
Procedure: 20 min.
Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. This method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.
抗原
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