Choline Assay Kit
BCA
Cell Culture Supernatant, Cell Culture Supernatant, Milk, Plasma, Saliva, Serum, Urine
1 image
(1 reference)
产品编号 ABIN1000299
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Choline Assay Kit (ABIN1000299)
抗原
Choline
应用范围
Biochemical Assay (BCA)
样品类型
Cell Culture Supernatant, Cell Culture Supernatant, Milk, Plasma, Saliva, Serum, Urine
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产品特性
- Use 20 µL samples. Linear detection range: colorimetric assay 1 to 100 µM, fluorimetric assay 0.2 to 10 µM choline.
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组件
- Assay Buffer: 10 mL. Enzyme Mix: 120 µL. Dye Reagent: 120 µL. Standard: 400 µL 2 mM Choline.
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试剂未包括
- Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.
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应用备注
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Assays: choline in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture.
Drug Discovery/Pharmacology: effects of drugs on choline metabolism. -
结果分析
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Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.
Conversions: 1 mM choline equals 10.4 mg/dL, 0.010% or 104 ppm. -
限制
- 仅限研究用
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储存条件
- -20 °C
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: "Carnitine modulates crucial myocardial adenosine triphosphatases and acetylcholinesterase enzyme activities in choline-deprived rats." in: Canadian journal of physiology and pharmacology, Vol. 92, Issue 1, pp. 78-84, (2014) (PubMed).
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: "Carnitine modulates crucial myocardial adenosine triphosphatases and acetylcholinesterase enzyme activities in choline-deprived rats." in: Canadian journal of physiology and pharmacology, Vol. 92, Issue 1, pp. 78-84, (2014) (PubMed).
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- Choline
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背景
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Quantitative determination of choline by colorimetric (570nm) or fluorometric (530nm/590nm) methods.
Procedure: 30 min.
Choline and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety. This method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to betaine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.
抗原
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