Acetylcholine Assay Kit
(6 references)-
- 抗原 See all Acetylcholine (ACH) 试剂盒
- Acetylcholine (ACH)
- 应用范围
- Biochemical Assay (BCA)
- 样品类型
- Cell Culture Supernatant, Cell Culture Supernatant, Milk, Plasma, Saliva, Serum, Urine
- 产品特性
- Use 20 µL samples. Linear detection range: colorimetric assay 10 to 200 µM, fluorimetric assay 0.4 to 10 µM acetylcholine.
- 组件
- Assay Buffer: 10 mL. ACHE Enzyme: 120 µL. Enzyme Mix: 120 µL. Dye Reagent: 120 µL. Standard: 400 µL 2 mM acetylcholine.
- 试剂未包括
- Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.
- Top Product
- Discover our top product ACH ELISA Kit
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Acetylcholine (ACH) ELISA Kit
ACH 适用: Various Species Colorimetric Competition ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Acetylcholine (ACH) ELISA KitACH 适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
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- 应用备注
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Assays: acetylcholine in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture.
Drug Discovery/Pharmacology: effects of drugs on acetylcholine metabolism. - 结果分析
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Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
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: "Total isoflavones from soybean and tempeh reversed scopolamine-induced amnesia, improved cholinergic activities and reduced neuroinflammation in brain." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 65, pp. 120-8, (2014) (PubMed).
: "Effects of the total alkaloidal extract of Murraya koenigii leaf on oxidative stress and cholinergic transmission in aged mice." in: Phytotherapy research : PTR, Vol. 27, Issue 1, pp. 46-53, (2013) (PubMed).
: "Withania somnifera root extract ameliorates hypobaric hypoxia induced memory impairment in rats." in: Journal of ethnopharmacology, Vol. 145, Issue 2, pp. 431-41, (2013) (PubMed).
: "Embryonic exposure to benzo(a)pyrene influences neural development and function in rockfish (Sebastiscus marmoratus)." in: Neurotoxicology, Vol. 33, Issue 4, pp. 758-62, (2012) (PubMed).
: "Exposure of Sebastiscus marmoratus embryos to pyrene results in neurodevelopmental defects and disturbs related mechanisms." in: Aquatic toxicology (Amsterdam, Netherlands), Vol. 116-117, pp. 109-15, (2012) (PubMed).
: "Acute exposure to DE-71: effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae." in: Environmental toxicology and chemistry / SETAC, Vol. 31, Issue 10, pp. 2338-44, (2012) (PubMed).
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: "Total isoflavones from soybean and tempeh reversed scopolamine-induced amnesia, improved cholinergic activities and reduced neuroinflammation in brain." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 65, pp. 120-8, (2014) (PubMed).
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- 抗原
- Acetylcholine (ACH)
- 别名
- Acetylcholine (ACH 产品)
- 物质类
- Chemical
- 背景
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Quantitative determination of acetylcholine by colorimetric (570nm) or fluorimetric (530nm/590nm) methods.
Procedure: 30 min.
Acetylcholine is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory and mood. This method provides a simple, direct and high- throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the acetylcholine concentration in the sample.
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