Human IgM isotype control (Biotin)
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Quick Overview for Human IgM isotype control (Biotin) (ABIN964511)
抗原
标记
应用范围
亚型
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宿主
- Human
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Supplier Product No.
- 009-0607
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Supplier
- Rockland
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原理
- Human IgM (myeloma) Whole Molecule Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgM and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Pepsin.
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产品特性
- Human IgM whole molecule Biotin conjugated, human myeloma IgM whole molecule biotin conjugation
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纯化方法
- This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation, ion exchange chromatography followed by tandem molecular sieve chromatography and extensive dialysis against the buffer stated above.
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Rat IgM isotype control
FACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Rabbit IgM isotype controlIsoC, ELISA, IHC Polyclonal IgM unconjugated
Mouse IgM isotype control (Biotin)FACS, ELISA Monoclonal 11E10 IgM Biotin
Rat IgM isotype controlFACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Mouse IgM isotype controlFACS, ELISA Monoclonal 11E10 IgM unconjugated
Mouse IgM isotype controlIsoC, FACS, ELISA, IHC Monoclonal 11E10 IgM unconjugated
Rat IgM isotype control (Biotin)FACS, ELISA Monoclonal NIP-M-2 IgM Biotin
Rat IgM isotype control (FITC)FACS, ELISA Monoclonal NIP-M-2 IgM FITC
Mouse IgM isotype control (FITC)FACS, ELISA Monoclonal 11E10 IgM FITC
Mouse IgM isotype controlIsoC, FACS, ELISA, WB, IHC (p), IHC (fro), ICC, IP Monoclonal PFR-03 IgM unconjugated
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应用备注
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Application Note: Human IgM (myeloma) Biotin conjugated whole molecule can be used as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgM
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物质类
- Antibody
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背景
- Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
抗原
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