Human IgM isotype control (HRP)
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北京 101111
Quick Overview for Human IgM isotype control (HRP) (ABIN964509)
抗原
片段
标记
应用范围
亚型
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宿主
- Human
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Supplier Product No.
- 009-0309
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Supplier
- Rockland
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原理
- Human IgM (myeloma) Fab μ Fragment Peroxidase Conjugated
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交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Human IgM, anti-Human IgG F(ab')2 and anti-Human Serum. No reaction was observed against anti-Human IgG F(c).
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产品特性
- Human IgM Fab μ peroxidase conjugation, Human myeloma IgM Fabμ HRP conjugation
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纯化方法
- This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by enzyme digestion and extensive dialysis against the buffer stated above.
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应用备注
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Application Note: HUMAN IgM (myeloma) Fab μ fragment peroxidase can be utilized in Western Blotting and ELISA experiments. Specific conditions should be optimized by the user.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgM
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物质类
- Antibody
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背景
- Background: Human IgM Fab mu Peroxidase is ideal for investigators involved in serum protein component research. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen. The spleen is the major site of specific IgM production. Distinct heavy chains differ in size and composition, alpha and gamma contain approximately 450 amino acids, while μ and ε have approximately 550 amino acids.
抗原
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