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Quick Overview for Human IgM 同型对照 (ABIN964504)
抗原
片段
应用范围
亚型
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宿主
- Human
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Supplier Product No.
- 009-0131
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Supplier
- Rockland
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原理
- Human IgM (myeloma) Fc5μ Fragment
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交叉反应 （详细）
- A single precipitin arc was observed against anti-human IgM Fc5μ and anti-human serum when assayed by immuno-electrophoresis at a concentration of 20 mg/ml. No reaction was observed against anti-Trypsin, anti-human IgG F(ab')2, anti-human IgG F(c), anti-human Kappa or anti-human Lambda.
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产品特性
- Human immunoglobulin M fragment, Human IgM Fc5μ
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纯化方法
- Human IgM (myeloma) Fc5μ fragment has been prepared from Human IgM myeloma protein by digestion with trypsin followed by column chromatography.
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纯度
- Purity was assessed by SDS-PAGE and HPLC to be greater than 95%.
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过滤
- Sterile filtered
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Rat IgM isotype control
FACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Rabbit IgM isotype controlIsoC, ELISA, IHC Polyclonal IgM unconjugated
Mouse IgM isotype control (Biotin)FACS, ELISA Monoclonal 11E10 IgM Biotin
Rat IgM isotype controlFACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Mouse IgM isotype controlFACS, ELISA Monoclonal 11E10 IgM unconjugated
Mouse IgM isotype controlIsoC, FACS, ELISA, IHC Monoclonal 11E10 IgM unconjugated
Rat IgM isotype control (Biotin)FACS, ELISA Monoclonal NIP-M-2 IgM Biotin
Rat IgM isotype control (FITC)FACS, ELISA Monoclonal NIP-M-2 IgM FITC
Mouse IgM isotype control (FITC)FACS, ELISA Monoclonal 11E10 IgM FITC
Mouse IgM isotype controlIsoC, FACS, ELISA, WB, IHC (p), IHC (fro), ICC, IP Monoclonal PFR-03 IgM unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Human IgM (myeloma) Fc5μ fragment has been tested in SDS-Page and can be used in Western Blotting and ELISA experiments as a control reagent.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- Optional[Buffer]: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 Preservative: 0.05 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. Human IgM (myeloma) Fc5μ fragment is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IgM
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物质类
- Antibody
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背景
- Background: Human IgM (myeloma) Fc5μ fragment consists of only the μ (mu) chain of the Fc fragment. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. IgM is formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum.
抗原
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