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Quick Overview for 小鸡 IgM 同型对照 (ABIN964487)
抗原
应用范围
亚型
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宿主
- 小鸡
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Supplier Product No.
- 003-0107
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Supplier
- Rockland
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原理
- Chicken IgM Whole Molecule
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交叉反应 （详细）
- Chicken IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and anti-Chicken IgM (μ chain specific). No reaction was observed against anti-Chicken IgG F(c). Some light chain cross reactivity will occur with anti-Chicken IgG. Analysis by SDS-PAGE was used to show purity at greater than 95%. Some high molecular weight banding may be visible. A minor band at ~50 kDa may also be visible.
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产品特性
- Chicken Immunoglobulin M, Fowl IgM
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纯化方法
- Chicken IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
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纯度
- >95 %
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过滤
- Sterile filtered
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Rat IgM isotype control
FACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Rabbit IgM isotype controlIsoC, ELISA, IHC Polyclonal IgM unconjugated
Mouse IgM isotype control (Biotin)FACS, ELISA Monoclonal 11E10 IgM Biotin
Rat IgM isotype controlFACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Mouse IgM isotype controlFACS, ELISA Monoclonal 11E10 IgM unconjugated
Mouse IgM isotype controlIsoC, FACS, ELISA, IHC Monoclonal 11E10 IgM unconjugated
Rat IgM isotype control (Biotin)FACS, ELISA Monoclonal NIP-M-2 IgM Biotin
Rat IgM isotype control (FITC)FACS, ELISA Monoclonal NIP-M-2 IgM FITC
Mouse IgM isotype control (FITC)FACS, ELISA Monoclonal 11E10 IgM FITC
Mouse IgM isotype controlIsoC, FACS, ELISA, WB, IHC (p), IHC (fro), ICC, IP Monoclonal PFR-03 IgM unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Chicken IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- Optional[Buffer]: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 Preservative: 0.1 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Chicken IgM whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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有效期
- 12 months
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- IgM
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物质类
- Antibody
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背景
- Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
抗原
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