小鼠 IgG3 isotype control (FITC)
Quick Overview for 小鼠 IgG3 isotype control (FITC) (ABIN964480)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
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宿主
- 小鼠
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Supplier Product No.
- 010-0243
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Supplier
- Rockland
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原理
- Mouse IgG3 Isotype Control Fluorescein Conjugated
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交叉反应 （详细）
- Typically less than 1% cross reactivity against other mouse heavy or light chain specific antibodies was detected by ELISA.
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产品特性
- Fluorescein conjugated Mouse IgG3 isotype control, Mouse IgG3 subclass isotype control FITC conjugation
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纯化方法
- This product has been prepared from serum-free tissue culture supernate by protein A chromatography.
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Labeling Ratio
- 3.3
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Mouse IgG3 isotype control (PE)
FACS, ELISA Monoclonal B10 IgG3 PE
Mouse IgG3 isotype control (FITC)FACS, ELISA Monoclonal B10 IgG3 FITC
Mouse IgG3 isotype control (APC)FACS, ELISA Monoclonal B10 IgG3 APC
Mouse IgG3 isotype control (SPRD)FACS, ELISA Monoclonal B10 IgG3 SPRD
Mouse IgG3 isotype control (Biotin)FACS, ELISA Monoclonal B10 IgG3 Biotin
Mouse IgG3 isotype control (PE-Cy7)FACS, ELISA Monoclonal B10 IgG3 PE-Cy7
Mouse IgG3 isotype controlFACS, ELISA Monoclonal B10 IgG3 unconjugated
Mouse IgG3 isotype controlFACS, ELISA, IsoC, IHC Monoclonal B10 IgG3 unconjugated
Mouse IgG3 isotype control (HRP)FACS, ELISA, FLISA Monoclonal B10 IgG3 HRP
Mouse IgG3 isotype control (Alkaline Phosphatase (AP))FACS, ELISA, FLISA Monoclonal B10 IgG3 Alkaline Phosphatase (AP)
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应用备注
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG3 isotype has been tested in dot blot and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 100 μL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG3
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物质类
- Antibody
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背景
- Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
抗原
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