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Quick Overview for 大鼠 IgG isotype control (HRP) (ABIN964390)
抗原
片段
标记
应用范围
亚型
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宿主
- 大鼠
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Supplier Product No.
- 012-0304
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Supplier
- Rockland
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原理
- Rat IgG F(ab')2 Fragment Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgG, anti-Rat IgG F(ab')2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.
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产品特性
- Rat IgG F(ab')2 fragment peroxidase conjugation, Rat IgG F(ab')2 fragment HRP conjugated
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纯化方法
- This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.
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Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Rabbit IgG isotype control (Atto 550)FACS, IHC Polyclonal IgG Atto 550
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
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应用备注
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Application Note: Rat IgG F(ab')2 Fragment Peroxidase Conjugated reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Thimerosal -
储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Background: Rat IgG F(ab')2 Fragment Peroxidase Conjugated is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . Rat IgG F(ab')2 Molecules lack the Fc portion of Rat IgG and therefore receptors that bind Rat IgG Fc will not bind Rat IgG F(ab')2 Molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region followed by Peroxidase conjugation.
抗原
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