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大鼠 IgG 同型对照

Rockland 012-0103 ELISA, WB, SDS, IHC IgG unconjugated Fc fragment
Rockland
产品编号 ABIN964381
Supplier Product No.: 012-0103
发货至: 中国
  • 抗原 See all IgG products
    IgG
    片段
    Fc fragment
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    大鼠
    应用范围
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Immunohistochemistry (IHC)
    Supplier Product No.
    012-0103
    Supplier
    Rockland
    原理
    Rat IgG Fc Fragment
    交叉反应 (详细)
    Rat IgG F(c) fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(c).  No reaction was observed against anti-Rat IgG F(ab')2 or anti-Papain.
    产品特性
    Rat Immunoglobulin G F(c) fragment, IgG Fc
    纯化方法
    Rat IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. 
    过滤
    Sterile filtered
    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: Rat IgG F(c) Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液
    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to opening. Stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景
    Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The F(c) fragment binds with very high affinity to the Fc receptor proteins on phagocytic leukocytes. When digested from the whole antibody molecule, the F(c) fragment no longer posses the epitope recognition site.
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