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Quick Overview for 兔 IgG isotype control (Biotin) (ABIN964369)
抗原
标记
应用范围
亚型
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宿主
- 兔
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Supplier Product No.
- 011-0602
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Supplier
- Rockland
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原理
- Rabbit IgG Whole Molecule Biotin Conjugated
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交叉反应 （详细）
- Rabbit IgG whole molecule Biotin conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit IgG and anti-Rabbit Serum.
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产品特性
- Rabbit IgG Biotin conjugation, Biotin conjugated Rabbit IgG whole molecule
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纯化方法
- Rabbit IgG whole molecule Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Rabbit IgG isotype control (Atto 550)FACS, IHC Polyclonal IgG Atto 550
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
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应用备注
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Application Note: Rabbit IgG whole molecule Biotin conjugated can be utilized as a control reagent in both Western Blotting and ELISA format experiments. A streptavidin-conjugated reporter (ie. Streptavidin Peroxidase Conjugated) can be used for detection.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Rabbit IgG whole molecule Biotin conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Rabbit IgG whole molecule Biotin Conjugated is ideal for investigators in Immunology, Cancer, and Microbiology research.
抗原
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