小鼠 IgG isotype control (Biotin)
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北京 101111
Quick Overview for 小鼠 IgG isotype control (Biotin) (ABIN964345)
抗原
标记
应用范围
亚型
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宿主
- 小鼠
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Supplier Product No.
- 010-0602
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Supplier
- Rockland
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原理
- Mouse IgG Whole Molecule Biotin Conjugated
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交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG and anti-Mouse Serum.
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产品特性
- Mouse IgG Whole Molecule Biotin conjugation
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纯化方法
- This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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应用备注
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Application Note: Mouse IgG Biotin can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Background: Mouse IgG purified protein (Immunoglobulin G) are antibody molecules. Mouse IgG is composed of four peptide chains - two heavy chains and two light chains. Mouse IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. Mouse IgG typically constitutes 75 % of serum immunoglobulins. Mouse IgG molecules are synthesized and secreted by plasma B cells. Ideal as a negative control for Flow Cytometry, Western blotting, immunoprecipitation and immunohistochemistry applications.
抗原
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