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Quick Overview for Human IgG isotype control (Biotin) (ABIN964324)
抗原
片段
标记
应用范围
亚型
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宿主
- Human
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Supplier Product No.
- 009-0603
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Supplier
- Rockland
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原理
- Human IgG Fc Fragment Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab')2 or anti-Papain.
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产品特性
- Human Immunoglobulin G F(c) fragment, IgG Fc, Vitamin H, Coenzyme R
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纯化方法
- Human IgG F(c) fragment Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
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Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Rabbit IgG isotype control (Atto 550)FACS, IHC Polyclonal IgG Atto 550
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:500
Application Note: Human IgG F(c) fragment Biotin conjugated has been tested in dot blot and can be utilized as a control reagent in both Western Blotting and ELISA format experiments.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:5000 - 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Human IgG F(c) fragment Biotin conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Background: Human IgG Fc purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of Human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
抗原
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