Human IgG isotype control (HRP)
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北京 101111
Quick Overview for Human IgG isotype control (HRP) (ABIN964318)
抗原
标记
应用范围
亚型
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宿主
- Human
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Supplier Product No.
- 009-0302
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Supplier
- Rockland
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原理
- Human IgG Whole Molecule Peroxidase Conjugated
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交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human IgG and anti-Human Serum.
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产品特性
- Human IgG peroxidase conjugated, Human IgG HRP conjugated
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纯化方法
- IgG was prepared from normal human serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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应用备注
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Application Note: Human IgG Whole Molecule Peroxidase Conjugated Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Background: Human IgG purified protein (Immunoglobulin G) are antibody molecules. Human IgG is composed of four peptide chains - two heavy chains gamma and two light chains. Human IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. Human IgG typically constitutes 75 % of serum immunoglobulins. Human IgG molecules are synthesized and secreted by plasma B cells.
抗原
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