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Armenian Hamster IgG 同型对照

FACS, SDS, FLISA, FM IgG unconjugated
Rockland
产品编号 ABIN964166
Supplier Product No.: 019-001-002
发货至: 中国
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Quick Overview for Armenian Hamster IgG 同型对照 (ABIN964166)

抗原

IgG

应用范围

Flow Cytometry (FACS), SDS-PAGE (SDS), FLISA, Fluorescence Microscopy (FM)

亚型

IgG
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    Armenian Hamster

    Supplier Product No.

    019-001-002

    Supplier

    Rockland

    原理

    Armenian Hamster IgG Whole Molecule Isotype Control

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Armenian Hamster IgG.  Greatly diminished reactivity will occur against anti-Golden Syrian Hamster IgG.

    产品特性

    Armenian Hamster IgG isotype Control, A. Hamster IgG control, Control Protein, Isotype Protein

    纯化方法

    ARMENIAN HAMSTER IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. 
  • 应用备注

    Flow Cytometry Dilution: 1:1000-1:5000

    Application Note: ARMENIAN HAMSTER IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by user.

    FLISA Dilution: User Optimized

    IF Microscopy Dilution: User Optimized

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Reconstitution Volume: 1.0 mL

    浓度

    1.0 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    IgG

    物质类

    Antibody

    背景

    Background: Armenian Hamster isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In hamster there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda.
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