Human IgA isotype control (FITC)
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北京 101111
Quick Overview for Human IgA isotype control (FITC) (ABIN964160)
抗原
标记
应用范围
亚型
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宿主
- Human
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Supplier Product No.
- 009-0206
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Supplier
- Rockland
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原理
- Human IgA Whole Molecule Fluorescein Conjugated
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交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human IgA and anti-Human Serum. No reaction was observed against anti-Human IgM Fc5μ or anti-Human IgG F(c).
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产品特性
- Human IgA fluorescein conjugated, Human IgA FITC conjugation
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纯化方法
- This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by extensive dialysis against the buffer stated above.
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Labeling Ratio
- 4.2
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应用备注
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Application Note: Human IgA whole molecule Fluorescein has been tested in dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgA
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物质类
- Antibody
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背景
- Background: Immunoglobulin A is the primary responder in muscosal immunity, and comprises close to 75 % of the total immunoglobulin produced. IgA can also be secreted and is protected from degradation by many proteolytic enzymes (which allows it to be secreted along the gastrointestinal tract). Immunoglobulin A only weakly activates the complement system and is not readily opsonized.
抗原
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