Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PACBLU, and AF488 conjugates 1 g/106 cells PE, APC, AF647 and AF700 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L FLISA FITC and AF488 conjugates 1:200 - 1:600 PE, APC, and AF647 conjugates 1 g/mL