Human IgM 同型对照
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北京 101111
Quick Overview for Human IgM 同型对照 (ABIN5633258)
抗原
片段
应用范围
亚型
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宿主
- Human
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Supplier Product No.
- 009-0109-0001
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Supplier
- Rockland
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原理
- Human IgM Fab μ Fragment
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum. No reaction was observed against anti-Human IgG F(c). Some light chain cross reactivity will occur with anti-Human IgG.
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产品特性
- Human Myeloma Fragment, fab fragment of a human
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纯化方法
- HUMAN IgM (myeloma) Fab μ fragment was prepared from serum by a multi-step process which includes delipidation, selective precipitation, tandem molecular sieve chromatography and trypsin digestion followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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Rabbit IgM isotype control
IsoC, ELISA, IHC Polyclonal IgM unconjugated
Mouse IgM isotype controlIsoC, FACS, ELISA, IHC Monoclonal 11E10 IgM unconjugated
Mouse IgM isotype control (PE)FACS, ELISA Monoclonal 11E10 IgM PE
Mouse IgM isotype control (APC)FACS, ELISA Monoclonal 11E10 IgM APC
Mouse IgM isotype control (PE-Cy7)FACS, ELISA Monoclonal 11E10 IgM PE-Cy7
Mouse IgM isotype control (SPRD)FACS, ELISA Monoclonal 11E10 IgM SPRD
Mouse IgM isotype control (FITC)FACS, ELISA Monoclonal 11E10 IgM FITC
Mouse IgM isotype control (FITC)IsoC, FACS Monoclonal PFR-03 IgM FITC
Mouse IgM isotype controlIsoC, FACS, ELISA, WB, IHC (p), IHC (fro), IP, ICC Monoclonal PFR-03 IgM unconjugated
Rat IgM isotype control (APC)FACS, ELISA Monoclonal NIP-M-2 IgM APC
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应用备注
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Application Note: Monovalent Fab fragments of affinity-purified, secondary antibodies are offered to cover (block) the surface of immunoglobulins for double labeling primary antibodies from the same host species, or to block endogenous immunoglobulins in tissue sections or on cell surfaces. They can be used for these purposes because each Fab fragment has only a single antigen binding site. HUMAN IgM (myeloma) Fab μ fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by the user.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IgM
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物质类
- Antibody
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背景
- Background: Human IgM Fab mu is ideal for investigators involved in serum protein component research. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen. The spleen is the major site of specific IgM production. Distinct heavy chains differ in size and composition, alpha and gamma contain approximately 450 amino acids, while μ and ε have approximately 550 amino acids.
抗原
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