Human IgM 同型对照
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- 抗原 See all IgM products
- IgM
- 片段
- Fab fragment
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宿主
- Human
- 应用范围
- ELISA, Western Blotting (WB)
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum. No reaction was observed against anti-Human IgG F(c). Some light chain cross reactivity will occur with anti-Human IgG.
- 纯化方法
- HUMAN IgM (myeloma) Fab μ fragment was prepared from serum by a multi-step process which includes delipidation, selective precipitation, tandem molecular sieve chromatography and trypsin digestion followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 亚型
- IgM
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- 应用备注
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Application Note: Monovalent Fab fragments of affinity-purified, secondary antibodies are offered to cover (block) the surface of immunoglobulins for double labeling primary antibodies from the same host species, or to block endogenous immunoglobulins in tissue sections or on cell surfaces. They can be used for these purposes because each Fab fragment has only a single antigen binding site. HUMAN IgM (myeloma) Fab μ fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by the user.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 别名
- IgM constant region Isotype Control, IGM Isotype Control
- 物质类
- Antibody
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