绵羊 IgG 同型对照
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北京 101111
Quick Overview for 绵羊 IgG 同型对照 (ABIN5633250)
抗原
片段
应用范围
亚型
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宿主
- 绵羊
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Supplier Product No.
- 013-0104-0002
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Supplier
- Rockland
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原理
- Sheep IgG F(ab')2 Fragment
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep IgG, anti-Sheep IgG F(ab')2 and anti-Sheep Serum. No reaction was observed against anti-Sheep IgG F(c) or anti-Pepsin.
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产品特性
- Sheep IgG F(ab')2 Fragment
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纯化方法
- This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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Hamster IgG isotype control
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Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
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应用备注
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Application Note: Sheep IgG F(ab')2 Fragment has been tested in SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Background: Sheep IgG F(ab')2 Fragment is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . Sheep IgG F(ab')2 Molecules lack the Fc portion of Sheep IgG and therefore receptors that bind Sheep IgG Fc will not bind Sheep IgG F(ab')2 Molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
抗原
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