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- 抗原 See all IgG products
- IgG
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宿主
- 大鼠
- 应用范围
- Flow Cytometry (FACS), ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Supplier Product No.
- 012-0102-0005
- Supplier
- Rockland
- 原理
- Rat IgG Whole Molecule
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat Serum.
- 产品特性
- Rat Immunoglobulin G, Rat IgG whole molecule, Rat IgG purified protein
- 纯化方法
- Rat IgG was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 亚型
- IgG
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Rabbit IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype control (PE)FACS, ELISA Polyclonal IgG PE
Goat IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
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- 应用备注
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Flow Cytometry Dilution: User Optimized
Application Note: Rat IgG whole molecule has been tested by SDS-Page and Western Blot and is suitable for use as antigen or ligand in immunochemical reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 500 μL
- 浓度
- 10.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Background: Rat IgG purified protein (Immunoglobulin G) are antibody molecules. Rat IgG is composed of four peptide chains - two heavy chains (gamma) and two light chains. Rat IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. Rat IgG typically constitutes 75 % of serum immunoglobulins. Rat IgG molecules are synthesized and secreted by plasma B cells.
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