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小鸡 IgG 同型对照

ELISA, WB, SDS, IHC IgG unconjugated
Rockland
产品编号 ABIN5633221
Supplier Product No.: 003-0102-0005
发货至: 中国

Quick Overview for 小鸡 IgG 同型对照 (ABIN5633221)

抗原

IgG

应用范围

ELISA, Western Blotting (WB), SDS-PAGE (SDS), Immunohistochemistry (IHC)

亚型

IgG
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    小鸡

    Supplier Product No.

    003-0102-0005

    Supplier

    Rockland

    原理

    Chicken IgG Whole Molecule

    交叉反应 (详细)

    Chicken IgG whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG and anti-Chicken Serum.

    产品特性

    Chicken immunoglobulin G, Chicken IgG, Chicken IgY

    纯化方法

    Chicken IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed extensive dialysis against the buffer stated above. 
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: Chicken IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Reconstitution Volume: 500 μL

    浓度

    10.0 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to restoration.   Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    IgG

    物质类

    Antibody

    背景

    Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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