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Quick Overview for Llama IgG isotype control (Biotin) (ABIN5608818)
抗原
标记
应用范围
亚型
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宿主
- Llama
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Supplier Product No.
- 025-0644
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Supplier
- Rockland
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原理
- Llama IgG2 Isotype Control Biotin Conjugated
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产品特性
- Llama IgG2 isotype, Llama IgG2 subclass isotype, Llama IgG2a, IgG2b, IgG2c, Llama IgG2 Biotin Conjugated, Llama IgG2 BAC, Llama isotype control
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纯化方法
- Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced unconjugated llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to unconjugated llama IgG1 or IgG3 are observed. Biotin Conjugated Llama IgG2 was proven by Dot Blot.
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Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Rabbit IgG isotype control (Atto 550)FACS, IHC Polyclonal IgG Atto 550
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
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应用备注
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Flow Cytometry Dilution: User Optimized
Application Note: Llama IgG2 Biotin conjugated isotype control has been tested by dot blot and can be utilized in Flow Cytometry, ELISA, and western blotting experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 100 μL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Background: Comparative studies of old world camelids (Camelus bactrianus and Camelus dromedarius) and new world camelids (Lama pacos, Lama glama and Lama vicugna) have shown that heavy-chain-only immunoglobulins represent between 35 % - 70 % of total IgG in the sera of all species. Such antibodies are homodimers of heavy chains that lack the CH1 domain of conventional antibodies and therefore do not interact with light chains, exhibiting a lower molecular weight ~100 kDa. In llama and other species of camelids, these heavy-chain-only immunoglobulins belong to the IgG2 and IgG3 subclasses of gamma chain antibodies.
抗原
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