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Quick Overview for 小鼠 IgG2b isotype control (APC) (ABIN5608811)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
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宿主
- 小鼠
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Supplier Product No.
- 010-2642
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Supplier
- Rockland
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原理
- Mouse IgG2b Isotype Control Allophycocyanin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Allophycoerythrin Conjugated Mouse IgG2b was proven by Dot Blot.
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产品特性
- allophycocyanin conjugated Mouse Isotype control, APC control, mouse IgG2b subclass isotype control
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纯化方法
- Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.
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过滤
- Sterile filtered
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Labeling Ratio
- 1-2
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Mouse IgG2b isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa Biotin
Mouse IgG2b isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa unconjugated
Mouse IgG2b isotype control (FITC)FACS, IsoC Monoclonal MPC-11 IgG2b kappa FITC
Mouse IgG2b isotype controlFACS, ELISA Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype control (PerCP)FACS, IsoC Monoclonal MPC-11 IgG2b kappa PerCP
Mouse IgG2b isotype control (APC)FACS, IsoC Monoclonal MPC-11 IgG2b kappa APC
Mouse IgG2b isotype control (PE)FACS, IsoC Monoclonal MPC-11 IgG2b kappa PE
Mouse IgG2b isotype control (iFluor™700)GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™700
Mouse IgG2b isotype control (iFluor™647)GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™647
Mouse IgG2b isotype control (iFluor™488)GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™488
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应用备注
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG2b kappa isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
FLISA Dilution: User Optimized
ELISA Dilution: 1:2000 - 1:20,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- Optional[Buffer]: None Preservative: 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
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有效期
- 12 months
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- IgG2b
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物质类
- Antibody
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背景
- Background: Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda.
抗原
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