小鼠 IgG2b isotype control (Biotin)
(5 references)Quick Overview for 小鼠 IgG2b isotype control (Biotin) (ABIN2749091)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
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宿主
- 小鼠
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原理
- Mouse IgG2b Isotype Control Biotin
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特异性
- This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
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无交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
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免疫原
- KLH-coupled trinitrophenol
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Mouse IgG2b isotype control
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa unconjugated
Mouse IgG2b isotype control (FITC)FACS, IsoC Monoclonal MPC-11 IgG2b kappa FITC
Mouse IgG2b isotype controlFACS, ELISA Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype control (PerCP)FACS, IsoC Monoclonal MPC-11 IgG2b kappa PerCP
Mouse IgG2b isotype control (APC)FACS, IsoC Monoclonal MPC-11 IgG2b kappa APC
Mouse IgG2b isotype control (PE)FACS, IsoC Monoclonal MPC-11 IgG2b kappa PE
Mouse IgG2b isotype control (iFluor™700)GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™700
Mouse IgG2b isotype control (iFluor™647)GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™647
Mouse IgG2b isotype control (iFluor™488)GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™488
Rat IgG2b isotype controlFACS, ELISA Monoclonal KLH-G2b-1-2 IgG2b unconjugated
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应用备注
- Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid ..." in: Veterinary research, Vol. 42, Issue 1, pp. 95, (2011) (PubMed).
: "Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells." in: World journal of gastroenterology, Vol. 16, Issue 5, pp. 618-24, (2010) (PubMed).
: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." in: Blood, Vol. 111, Issue 10, pp. 5037-46, (2008) (PubMed).
: "Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5226-35, (2006) (PubMed).
: "Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14012-20, (1999) (PubMed).
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: "Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid ..." in: Veterinary research, Vol. 42, Issue 1, pp. 95, (2011) (PubMed).
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- IgG2b
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别名
- IgG2b kappa
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物质类
- Antibody
抗原
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