HEK-293 Cells Immunoglobulin G4 (IgG4) 同型对照

ABIN2181283 产品详细信息, 供应商: Log in to see
  • immunoglobulin heavy constant gamma 4 (G4m marker)
  • IGHG4

HEK-293 Cells
Isotype Control (IsoC)
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Supplier Product No.
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亚型 IgG4
产品特性 This protein carries no "tag". The protein has a calculated MW of 25.8 kDa. As a result of glycosylation, the protein migrates as 33-35 kDa under reducing (R) condition, and 50-55 kDa under non-reducing (NR) condition (SDS-PAGE).
纯度 >95 % as determined by SDS-PAGE.
过滤 0.22 μm filtered
内毒素水平 Less than 1.0 EU per μg by the LAL method.
别名 IgG4
背景 Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).
分子量 25.8 kDa
UniProt P01861
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
状态 Lyophilized
溶解方式 Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
缓冲液 50 mM Tris, 100 mM Glycine, pH 7.5
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
SDS-PAGE (SDS) image for HEK-293 Cells Immunoglobulin G4 (IgG4) isotype control (ABIN2181283) Human IgG4 Fc, Tag Free on SDS-PAGE under reducing (R) and no-reducing (NR) condition...
背景 Chen, Jiang, Sun, Han, Wang, Ye, Wang, Zou: "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." in: Journal of proteome research, Vol. 8, Issue 2, pp. 651-61, 2009 (PubMed).

Kristiansen, Bunkenborg, Gronborg, Molina, Thuluvath, Argani, Goggins, Maitra, Pandey: "A proteomic analysis of human bile." in: Molecular & cellular proteomics : MCP, Vol. 3, Issue 7, pp. 715-28, 2004 (PubMed).

Frangione, Milstein, Pink: "Structural studies of immunoglobulin G." in: Nature, Vol. 221, Issue 5176, pp. 145-8, 1970 (PubMed).

ODonnell, Frangione, Porter: "The disulphide bonds of the heavy chain of rabbit immunoglobulin G." in: The Biochemical journal, Vol. 116, Issue 2, pp. 261-8, 1970 (PubMed).