Quick Overview for HEK-293 Cells IgG3 同型对照 (ABIN2181279)
抗原
应用范围
亚型
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适用
- 人
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宿主
- HEK-293 Cells
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产品特性
- Human IgG3-Fc / IGHG3 Region has a calculated MW of 31.2 kDa. The predicted N-terminus is Glu 99. The reducing (R) protein migrates as 40-50 kDa in SDS-PAGE due to glycosylation.
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纯度
- >95 % as determined by SDS-PAGE.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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Mouse IgG3 isotype control (PE)
FACS, ELISA Monoclonal B10 IgG3 PE
Mouse IgG3 isotype control (FITC)FACS, ELISA Monoclonal B10 IgG3 FITC
Mouse IgG3 isotype control (APC)FACS, ELISA Monoclonal B10 IgG3 APC
Mouse IgG3 isotype control (SPRD)FACS, ELISA Monoclonal B10 IgG3 SPRD
Mouse IgG3 isotype control (Biotin)FACS, ELISA Monoclonal B10 IgG3 Biotin
Mouse IgG3 isotype control (PE-Cy7)FACS, ELISA Monoclonal B10 IgG3 PE-Cy7
Mouse IgG3 isotype controlFACS, ELISA Monoclonal B10 IgG3 unconjugated
Mouse IgG3 isotype controlFACS, ELISA, IsoC, IHC Monoclonal B10 IgG3 unconjugated
Mouse IgG3 isotype control (HRP)FACS, ELISA, FLISA Monoclonal B10 IgG3 HRP
Mouse IgG3 isotype control (Alkaline Phosphatase (AP))FACS, ELISA, FLISA Monoclonal B10 IgG3 Alkaline Phosphatase (AP)
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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缓冲液
- 50 mM tris, 100 mM glycine, pH 7.5
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
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- IgG3
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物质类
- Antibody
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背景
- Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc / IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).
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分子量
- 31.2 kDa
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UniProt
- P01860
抗原
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