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HEK-293 Cells IgG2a 同型对照

IsoC IgG2a unconjugated
产品编号 ABIN2181277
发货至: 中国
  • 抗原 See all IgG2a products
    IgG2a
    适用
    小鼠
    宿主
    • 171
    • 66
    • 2
    • 1
    HEK-293 Cells
    应用范围
    Isotype Control (IsoC)
    产品特性
    This protein carries no "tag". The protein has a calculated MW of 26.4 kDa. As a result of glycosylation, the protein migrates as 25-30 kDa under reducing (R) condition, and 50-60 kDa under non-reducing (NR) condition (SDS-PAGE).
    纯度
    >90 % as determined by SDS-PAGE.
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1.0 EU per μg by the LAL method.
    亚型
    IgG2a
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
    缓冲液
    50 mM Tris, 100 mM Glycine, pH 7.5
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
  • 抗原
    IgG2a
    Abstract
    IgG2a 产品
    别名
    gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
    物质类
    Antibody
    背景
    Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
    分子量
    26.4 kDa
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