HEK-293 Cells Immunoglobulin G2a (IgG2a) 同型对照

ABIN2181277 产品详细信息, 供应商: Log in to see
  • gamma-2a immunoglobulin heavy chain
  • IgG-2a
HEK-293 Cells
Isotype Control (IsoC)
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Supplier Product No.
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亚型 IgG2a
产品特性 This protein carries no "tag". The protein has a calculated MW of 26.4 kDa. As a result of glycosylation, the protein migrates as 25-30 kDa under reducing (R) condition, and 50-60 kDa under non-reducing (NR) condition (SDS-PAGE).
纯度 >95 % as determined by SDS-PAGE.
过滤 0.22 μm filtered
内毒素水平 Less than 1.0 EU per μg by the LAL method.
别名 IgG2a
背景 Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
分子量 26.4 kDa
UniProt P01863
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
状态 Lyophilized
溶解方式 Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
缓冲液 50 mM Tris, 100 mM Glycine, pH 7.5
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
SDS-PAGE (SDS) image for HEK-293 Cells Immunoglobulin G2a (IgG2a) isotype control (ABIN2181277) Mouse IgG2a Fc, Tag Free on SDS-PAGE under reducing (R) and no-reducing (NR) conditio...
背景 Sikorav, Auffray, Rougeon: "Structure of the constant and 3' untranslated regions of the murine Balb/c gamma 2a heavy chain messenger RNA." in: Nucleic acids research, Vol. 8, Issue 14, pp. 3143-55, 1981 (PubMed).

Ollo, Auffray, Morchamps, Rougeon: "Comparison of mouse immunoglobulin gamma 2a and gamma 2b chain genes suggests that exons can be exchanged between genes in a multigenic family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, Issue 4, pp. 2442-6, 1981 (PubMed).