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小鼠 IgG2a isotype control (PE)

FACS, IsoC, IF IgG2a PE
产品编号 ABIN2145401
发货至: 中国
  • 抗原 See all IgG2a products
    IgG2a
    宿主
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    小鼠
    标记
    PE
    应用范围
    Flow Cytometry (FACS), Isotype Control (IsoC), Immunofluorescence (IF)
    产品特性
    This antibody can be used as a mouse IgG2a isotype control in flow cytometry and other applications.
    亚型
    IgG2a
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 20 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
  • 抗原
    IgG2a
    Abstract
    IgG2a 产品
    别名
    gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
    物质类
    Antibody
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