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Quick Overview for 小鼠 IgG2b isotype control (PE) (ABIN1045090)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
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宿主
- 小鼠
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Supplier Product No.
- 010-0842
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Supplier
- Rockland
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原理
- Mouse IgG2b Isotype Control Phycoerythrin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Phycoerythrin Conjugated Mouse IgG2b was proven by Dot Blot.
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产品特性
- Phycoerythrin conjugation Mouse IgG2b isotype control, Mouse IgG2b subclass isotype control PE conjugated
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纯化方法
- This product was prepared from ascitic fluid by affinity chromatography.
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Mouse IgG2b isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b Biotin
Mouse IgG2b isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b unconjugated
Mouse IgG2b isotype control (FITC)FACS, IsoC Monoclonal MPC-11 IgG2b FITC
Mouse IgG2b isotype controlFACS, ELISA Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype control (APC)FACS, IsoC Monoclonal MPC-11 IgG2b APC
Mouse IgG2b isotype control (PE)FACS, IsoC Monoclonal MPC-11 IgG2b PE
Mouse IgG2b isotype control (PerCP)FACS, IsoC Monoclonal MPC-11 IgG2b PerCP
Mouse IgG2b isotype control (iFluor™700)GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™700
Mouse IgG2b isotype control (iFluor™647)GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™647
Mouse IgG2b isotype control (iFluor™488)GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™488
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应用备注
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Flow Cytometry Dilution: User Optimized
Application Note: Mouse IgG2b isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
FLISA Dilution: User Optimized
IF Microscopy Dilution: 1:1000-1:5000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
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有效期
- 12 months
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- IgG2b
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物质类
- Antibody
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背景
- Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
抗原
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