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Quick Overview for 豚鼠 IgG isotype control (Biotin) (ABIN1043594)
抗原
片段
标记
应用范围
亚型
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宿主
- 豚鼠
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Supplier Product No.
- 006-0603
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Supplier
- Rockland
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原理
- Guinea Pig IgG Fc Fragment Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Guinea Pig IgG, anti-Guinea Pig IgG F(c) and anti-Guinea Pig Serum. No reaction was observed against anti-Guinea Pig IgG F(ab')2 or anti-Papain.
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产品特性
- Guinea Pig IgG F(c) fragment Biotin conjugation, Guinea Pig IgG Fc fragment BAC conjugate
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纯化方法
- This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
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Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Rabbit IgG isotype control (Atto 550)FACS, IHC Polyclonal IgG Atto 550
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
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应用备注
- Optional[Flow Cytometry Dilution]: Guinea Pig IgG F(c) fragment Biotin conjugation has been tested in SDS-Page and dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging utilizing various commercial platforms.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This antibody possesses the F(c) region, recognized by high-affinity Fc receptor proteins.
抗原
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