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Z-VAD-FMK (General Caspase Inhibitor)

Name: Z-VAD-FMK (General Caspase Inhibitor)
SKU: ABIN2691062
Availability: OutOfStock

Func, CC, BR, InhA Cell-permeable 187389-52-2 Z-VAD fluoromethylketone

(2 references)

产品编号 ABIN2691062

发货至: 中国

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Quick Overview for Z-VAD-FMK (General Caspase Inhibitor) (ABIN2691062)

应用范围

Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)

Chemical Name

Z-VAD fluoromethylketone

Permeability

Cell-permeable

原理

A pan-caspase inhibitor

序列

Z-Val-Ala-Asp(OMe)-FMK

产品特性

Members of the caspase family play key roles in inflammation and mammalian apoptosis. Z-VAD-FMK is a cell permeable general caspase inhibitor that irreversibly binds to the catalytic site of caspase proteases and inhibits apoptosis. The peptide is O-methylated in the P1 position on aspartic acid providing enhanced stability and increased cell permeability. Z-VAD-FMK has a molecular weight of 467 Daltons. Flow cytometric analysis of apoptosis in Jurkat cells (Human T-cell leukemia, ATCC TIB-152). Jurkat cells were preincubated with the following: no inhibitor (upper left and bottom left panels), 20 μM Z-VAD-FMK (upper center and bottom center panels) or 20 μM negative control inhibitor (Z-FA-FMK, upper right and bottom right panels) (Cat. No. 550411) for 30 min, and then either left untreated (bottom row) or treated with 4 μM of campthothecin for 3 hr (top row). Following incubation, cells were collected and stained with PE Annexin V (Cat. No. 559763) to identify cells undergoing apoptosis. The results indicate that in campthothecin treated cells, approximately 42 % of the cells were induced to undergo apoptosis and the use of the general caspase inhibitor Z-VAD-FMK reduced the level of apoptosis to that observed in the untreated control. Cells treated with Z-FA-FMK (Cat. No. 550411) showed similar results to the campthothecin treated cells without inhibitor, indicating that the negative control inhibitor did not attenuate apoptosis.

纯化方法

Purified

Formula

C22H30N3O7F

Solubility

DMSO
Geldanamycin- FITC
Inh 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Geldanamycin
Inh 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Radicicol
Inh 12772-57-5

Geldanamycin- Biotin
Inh 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Herbimycin A
Inh 70563-58-5 [(2R,3S,5S,6R,7S,8E,10S,11S,12E,14E)-2,5,6,11-tetramethoxy-3,7,9,15-tetramethyl-16,20,22-trioxo-17-azabicyclo[16.3.1]docosa-1(21),8,12,14,18-pentaen-10-yl] carbamate

17-GMB-APA-GA
Inh [(3R,5S,6R,7S,10S,11S)-21-[3-[4-(2,5-dioxopyrrol-1-yl)butanoylamino]propylamino]-6-hydroxy-5,11-dimethoxy-3,7,9,15-tetramethyl-16,20,22-trioxo-17-azabicyclo[16.3.1]docosa-1(21),8,12,14,18-pentaen-10-yl] carbamate

TEMPO
Inh 2564-83-2 2,2,6,6-Tetramethylpiperidine 1-oxyl, 2,2,6,6-Tetramethyl-1-piperidinyloxy, free radical, (2,2,6,6-Tetramethyl-1-piperidinyl)oxidanyl, 1,1,5,5-tetramethylpentamethylene nitroxide, 2,2,6,6-Tetramethylpiperidine-1-oxyl

Triacsin C
Inh 76896-80-5 (3E)-1-Oxo-3-[(2E,4E,7E)-2,4,7-undecatrien-1-ylidene]triazane

TPCK
Inh 402-71-1 N-(4-Chloro-3-oxo-1-phenyl-2-butanyl)-4-methylbenzenesulfonamide

Staurosporine
Inh 62996-74-1 (2S,3R,4R,6R)-3-Methoxy-2-methyl-4-(methylamino)-29-oxa-1,7,17-triazaoctacyclo[12.12.2.12,6.07,28.08,13.015,19.020,27.021,26]nonacosa-8,10,12,14,19,21,23,25,27-nonaen-16-one

说明

BD Pharmingen™ Z-VAD-FMK, General Caspase Inhibitor – Purified
Members of the caspase family play key roles in inflammation and mammalian apoptosis. Z-VAD-FMK is a cell permeable general caspase inhibitor that irreversibly binds to the catalytic site of caspase proteases and inhibits apoptosis. The peptide is O-methylated in the P1 position on aspartic acid providing enhanced stability and increased cell permeability. Z-VAD-FMK has a molecular weight of 467 Daltons.

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitute the Z-VAD-FMK inhibitor in DMSO before use. The reconstituted Z-VAD-FMK inhibitor may be stored in small aliquots at -20 °C.

储存条件

-20 °C

储存方法

Avoid multiple freeze-thaws of product. Store the lyophilized Z-VAD-FMK inhibitor at -20°C. Reconstitute the Z-VAD-FMK inhibitor in DMSO before use. The reconstituted Z-VAD-FMK inhibitor may be stored in small aliquots at -20°C.
Gregoli, Bondurant: "Function of caspases in regulating apoptosis caused by erythropoietin deprivation in erythroid progenitors." in: Journal of cellular physiology, Vol. 178, Issue 2, pp. 133-43, (1999) (PubMed).

Thornberry, Lazebnik: "Caspases: enemies within." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1312-6, (1998) (PubMed).
分子量

467 Da

CAS-编号

187389-52-2