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Hub1 抗体

Hub1 适用: Saccharomyces cerevisiae WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN100576
发货至: 中国
  • 抗原
    Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))
    适用
    Saccharomyces cerevisiae
    宿主
    克隆类型
    多克隆
    应用范围
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    免疫原
    This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Hub1 protein.
    Immunogentype:Recombinant
    亚型
    IgG
  • 应用备注
    This purified polyclonal antibody reacts with yeast Hub1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates).  Other intrinsic bands are readily detectable at lower dilutions. For immunoblotting a 9.7 kDa band corresponding to yeast Hub1 is detected.  Most yeast cell lysates can be used as a positive control without induction or stimulation. 
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Restore with deionized water (or equivalent)
    浓度
    5.0 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
  • 抗原
    Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))
    别名
    Hub1
    背景
    Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.  Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs).  These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBLs, UDPs are not conjugated to other proteins.  Hub1 (''Homologous to UBiquitin'') may function as a modifier (see figure 2) but its role is unclear because it lacks the double glycine motif characteristic for ubiquitin and ubiquitin-like modifiers. Recently cell polarity factors Sph1 and Hbt1 have been identified as in vivo targets of Hub1 conjugation.
    Synonyms: Homologous to UBiquitin antibody, Hub1 protein antibody, HUB1 target protein 1 antibody, Hub1p antibody, Ubiquitin like protein modifier antibody
    基因ID
    855767, 6324360
    UniProt
    Q6Q546
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