HSP21 抗体
This anti-HSP21 antibody is a 兔 多克隆 antibody detecting HSP21 in WB. Suitable for Arabidopsis thaliana 和 Nicotiana tabacum. This Primary Antibody has been cited in 2+ publications.
(2 references)Quick Overview for HSP21 抗体 (ABIN3155734)
抗原
HSP21
(Heat Shock Protein 21 (HSP21))
适用
Arabidopsis thaliana, Nicotiana tabacum
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB)
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交叉反应 （详细）
- Not reactive in: Chlamydomonas reinhardtii
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预测反应
- Arabidopsis thaliana
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产品特性
- Expected / apparent Molecular Weight of the Antigene: 25 / 21 kDa
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纯化方法
- serum
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免疫原
- Recombinant Hsp21 protein derived from the sequence of Arabidopsis thaliana hsp21 P31170, At4g27670
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应用备注
- 1: 3000 with standard ECL (WB)
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说明
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Please, note that there might be no HSPs accumulation below temperature of 32-34°C. HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C. So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C.Another very effective parameter is the humidity. When using low humidity the plant has a chance to cool down through transpiration. In this case the HSPs induction requires higher temperatures.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water.
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注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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: "The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis." in: Plant physiology, Vol. 152, Issue 3, pp. 1471-83, (2010) (PubMed).
: "Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves." in: BMC plant biology, Vol. 10, pp. 34, (2010) (PubMed).
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: "The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis." in: Plant physiology, Vol. 152, Issue 3, pp. 1471-83, (2010) (PubMed).
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- HSP21 (Heat Shock Protein 21 (HSP21))
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别名
- HSP21
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背景
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AGI Code: At4g27670
Hsp21 is a chloroplast-localized small heat shock protein. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. -
分子量
- expected: 25 kDa, apparent: 21 kDa
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UniProt
- P31170
抗原
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