HIST1H3J Protein (His tag)
This Recombinant HIST1H3J protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for HIST1H3J Protein (His tag) (ABIN5505657)
抗原
HIST1H3J
(Histone Cluster 1, H3j (HIST1H3J))
蛋白类型
Recombinant
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Antibody Production (AbP), Standard (STD)
纯度
> 80 % as determined by SDS-PAGE and Coomassie blue staining
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标记
- This HIST1H3J protein is labelled with His tag.
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产品特性
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- Recombinant human Purified recombinant protein of Human histone cluster 1, H3j (HIST1H3J), full length, with N-terminal HIS tag, expressed in E.coli, 50 μg (full length, N-term HIS tag) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
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纯化方法
- Purified
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the N-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- HIST1H3J (Histone Cluster 1, H3j (HIST1H3J))
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别名
- histone cluster 1, H3j
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背景
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
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分子量
- 15.2 kDa
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NCBI登录号
- NP_003526
抗原
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