Heat Shock Protein 65 (HSP65) 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in EIA 和 WB. Suitable for Mycobacteria.
Quick Overview for Heat Shock Protein 65 (HSP65) 抗体 (ABIN2859572)
抗原
See all Heat Shock Protein 65 (HSP65) products
Heat Shock Protein 65 (HSP65)
适用
Mycobacteria
宿主
小鼠
克隆类型
单克隆
应用范围
Enzyme Immunoassay (EIA), Western Blotting (WB)
克隆位点
BDI578
-
-
特异性
- This antibody is reactive with Hsp65 (GroEL) of M. tuberculosis. Does not react with M. bovis, M. avium, M. phlei, M. parafortuitum, Rhodococcus sp., B. subtilis, S. pneumoniae, and E. coli.
-
产品特性
- Synonyms: 60 kDa chaperonin 2, Protein Cpn60-2, groEL protein 2, Cell wall protein A, Antigen A,groL2, groEL-2, groEL2, hsp65, Rv0440, MT0456, MTV037.04
-
纯化方法
- Protein A chromatography
-
纯度
- > 90 % pure
-
免疫原
- Purified protein derivative (PPD)
-
亚型
- IgG2a
-
-
-
应用备注
-
ELISA. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 0,1 mg/mL (OD280nm, E0.1% = 1.4)
-
缓冲液
- 0.01 M PBS, pH 7.2 containing 0.09 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
-
Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
-
-
- Heat Shock Protein 65 (HSP65)
-
别名
- Heat Shock Protein 65 / HSP65
-
背景
- Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure, most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.Synonyms: 60 kDa chaperonin 2, Antigen A, Cell wall protein A, MT0456, MTV037.04, Protein Cpn60-2, Rv0440, groEL protein 2, groEL-2, groEL2, groL2, hsp65
-
基因ID
- 886354
-
UniProt
- P0A520
-
途径
- Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
抗原
-
You are here:
